Az IPC technológia szépen fejlődik.
A legújabb eredmény ingyenesen is elérhető.
Ezúttal nem retrovirusokkal érték el a visszaprogramozást, hanem szintetikus mRNA bejutattásával. A hatékonyság kb 2-4%os. Igazából innen a fő akadályt már az őssejtkolóniák kultúrásában, minőség kontrolljában és irányitott differenciálásában látom.
Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA
Abstract
Clinical application of induced pluripotent stem cells
(iPSCs) is limited by the low efficiency of iPSC derivation
and the fact that most protocols modify the
genome to effect cellular reprogramming. Moreover,
safe and effective means of directing the fate of
patient-specific iPSCs toward clinically useful cell
types are lacking. Here we describe a simple, nonintegrating
strategy for reprogramming cell fate based
on administration of synthetic mRNA modified to
overcome innate antiviral responses. We show that
this approach can reprogram multiple human cell
types to pluripotency with efficiencies that greatly
surpass established protocols. We further show
that the same technology can be used to efficiently
direct the differentiation of RNA-induced pluripotent
stem cells (RiPSCs) into terminally differentiated
myogenic cells. This technology represents a safe,
efficient strategy for somatic cell reprogramming
and directing cell fate that has broad applicability
for basic research, disease modeling, and regenerative
medicine.